Extended Data Fig. 3: Induction of cell death by SEPHS2 KO in cancer cells.
From: Selenium detoxification is required for cancer-cell survival
a, Raw flow cytometry of PI and Annexin V double-stained U251 and MDAMB231 cells following KO with guides against SEPHS2 at 10 days after viral infection. Gating strategy is outlined in Extended Data Fig. 10a–e b, Percentages of Annexin V + /PI- and Annexin V + /PI + cells. c, Cleaved caspase 3 (CC3) staining in MDAMB231 and U251cells following KO with guides against SEPHS2 at 10 days after viral transduction. Scale bar=20μm. d, Quantification of CC3 positive cells over total cells in CTRL and SEPHS2 deficient MDAMB231 and U251. Counts are from random fields. e, Viability of control and SEPHS2 deficient U251 and MDAMB231 cells treated with vehicle or the indicated drugs for 4 days. Values are relative to the cell lines expressing nontargeting (CTRL) guide, set at 1.0. For e, n = 3 biological replicates; error bars are S.D. For all panels, the measure of center is mean. For all panels, *p < 0.05, **p < 0.01, and ***p < 0.001 (student’s two-tailed t test).
