Extended Data Fig. 7: Kir2.1 expression reduces neuron activity and related effects on energy balance.
From: Profound and redundant functions of arcuate neurons in obesity development
a, Vgat-Cre mice received either control vector (top panels) or AAV-DIO-Kir2.1-p2A-dTomato (bottom panels) to the Arc, and immunostraining was performed 4 weeks after viral delivery for c-Fos (green column) in response to overnight fasting (magnified pictures showing details of the boxed areas in the merged pictures as indicated), n = 3 as presented in panel b. b, Comparison in the number of Arc neurons with c-Fos expression between groups (n = 3 each/group, t = 3.648 df=4, **p = 0.0021, 2-sided unpaired Student’s t test). c-e, CLAMS measurements of feeding (c), O2 consumption (d) and locomotion (e) in mice with control or Kir2.1-vector delivery to bilateral Arc of Vgat-Cre mice. The CLAMS measurement was performed during the first week after viral delivery when body weight difference is minimal, n = 5 each/group as presented in Fig. 6g–i. f, AgRP-Cre mice received either control vector (top panels) or kir2.1 vectors (bottom panels) to the Arc, and immunostraining was performed 4 weeks after viral delivery for c-Fos (green column) in response to overnight fasting (magnified pictures on the right showing details of the boxed areas in the merged pictures as indicated), n = 3 as presented in panel g. g, Comparison in c-Fos neurons in response to fasting in the Arc between groups (n = 3 each/group, t = 3.648, df=4, **p = 0.0218, 2-sided Student’s t test). h–j, Comparison in food intake (h, n = 5 (control) or 7 (Kir2.1), t = 0.6790, df=10, p = 0.5125, 2-sided unpaired Student’s t test), fat mass (i, n = 5 (control) or 7 (Kir2.1), t = 1.507, df=10, p = 0.1628, 2-sided unpaired Student’s t test) and lean mass (j, n = 5 (control) or 7 (Kir2.1), t = 0.9326, df=10, p = 0.3730, 2-sided unpaired Student’s t test) during the 3rd week after viral delivery. 3 V: the third ventricle. Data presented as mean + /-sem.
