Extended Data Fig. 7: AMPKβ1N111 does not alter sensitivity to palmitoyl-CoA or AMP.
From: Long-chain fatty acyl-CoA esters regulate metabolism via allosteric control of AMPK β1 isoforms
AMPK activity of AMPKα1β1γ1 (WT, β1N111A or β1N111D) was determined by 32P SAMS assay, following immobilization on anti-myc agarose, ± palmitoyl-CoA (10 µM) or AMP (100 µM). Data are shown as mean fold change in activity vs. vehicle, ± s.e.m.; n = 3. Statistical significance was calculated using two-way ANOVA with Bonferroni’s multiple comparisons test. n represent biological independent experiments.
