Extended Data Fig. 1: Specificity of AMPK activation by palmitoyl-CoA and effects of co-incubations with free palmitate or coenzyme A.
From: Long-chain fatty acyl-CoA esters regulate metabolism via allosteric control of AMPK β1 isoforms
a, b, Activities of AMPKα1β1γ1 (Sf9 insect cell-expressed), determined by TR-FRET SAMS assay, in the presence of coenzymes (100 µM), cofactors (100 µM) and vitamins (100 µM) (a), or following 15 min pre-incubation in the presence of palmitoyl-CoA (10 µM) ± indicated concentrations of free palmitate or coenzyme A (b). Data are shown as mean fold change in AMPK activity vs. vehicle ± s.e.m. For a, n = 3 except for folic acid, thiamine, riboflavin, pyridoxine, biotin and niacin (n = 2); for b, n = 5 (palmitate incubation) or n = 3 (coenzyme A incubation). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparisons test. n represent biological independent experiments.
