Extended Data Fig. 3: Signalling timecourse during TGR.
From: Metabolic conditioning of CD8+ effector T cells for adoptive cell therapy
Protein isolates were prepared as described in (Fig. 3f), taking samples every 2 h over the 20 h exposure to limiting glucose concentration. Immunoblot analysis of protein extracts from equal cell numbers probed for phosphorylated acetyl-coA carboxylase at Ser79 (p-ACC1Thr79), total ACC1, phosphorylated AMPK at Thr172 (p-AMPkThr172), total AMPK, phosphorylated ribosomal protein S6 at Ser235/236 (p-S6Ser235/236), total S6, phosphorylated 4E-binding protein 1 at Thr37/46 (p-4E-BP1Thr37/46), total 4E-BP1, and Glut1. Tubulin was used as a loading control. Representative of 3 biological independent samples. Biological replicate data is shown in Fig. 3f.
