Extended Data Fig. 6: Antibiotics attenuate fructose- induced hepatosteatosis, barrier deterioration and other alterations in BL6 mice.
From: Fructose stimulated de novo lipogenesis is promoted by inflammation
a–g, Male BL6 mice were fed CSD or HFrD ± Abx. a, After five months, liver tissue sections were stained with ORO (n = 6 samples from different mice per group). Representative images are shown. Scale bars, 100 μm. b, c, Inflammatory and DNL-related mRNAs in liver were determined by Q-RT- PCR (n = 9 per group). d, e, Inflammatory and lipogenic proteins in liver lysates were analyzed by IB analysis (n = 7 protein lysates per group). Representative blots are shown. f, g, Q-RT-PCR analysis of indicated TJP genes in jejunum (f) and colon (g) (n = 9 per group). Two-sided Student’s t test was used to determine mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001. Benjamini- Hochberg FDR adjustment for P-values has been performed on data presented in panels b, c, f, g.
