Extended Data Fig. 1: HFrD stimulates HCC development without affecting body weight or white adipose tissue.
From: Fructose stimulated de novo lipogenesis is promoted by inflammation
a, Food consumption by MUP- uPA and BL6 mice given CSD (for MUP-uPA n = 4 cages/9 male mice; for BL6 n = 5 cages/ 11 male mice) or HFrD (for MUP-uPA n = 4 cages/12 male mice; for BL6 n = 5 cages/ 12 male mice). b, Formalin-fixed, paraffin-embedded (FFPE) and frozen liver tissue sections of DEN- challenged male BL6 mice were stained with H&E or ORO (n = 6 per group). Representative images are shown. Scale bars, 100 μm. c, Total triglycerides in livers of DEN- challenged male BL6 mice (n = 6 per group) are presented as fold-change relative to CSD-fed mice. d, e, Body and WAT weight of male MUP-uPA (n = 9 per group) (d) and DEN-challenged male BL6 (n = 11 for CSD group at 6 months and n = 9 for CSD group at 9 months; n=12 for HFrD group at 6 months and n=9 for HFrD group at 12 months) (e) mice given CSD or HFrD were measured at the indicated time points. f, Colon length in indicated mice (MUP-uPA n = 9, BL6 n=12, per group). g, h, MUP-uPA mice (n = 15 per group) (g) or DEN- challenged BL6 mice (n = 14 per group) (h) fed HFrD (▲) or CSD (■) for 5 months were subjected to glucose tolerance tests (GTT). i, j, CSD- or HFrD- fed MUP-uPA mice (n = 4 male mice per group) (i) or DEN-challenged BL6 mice (n = 6 male mice per group) (j) were placed in metabolic cages for two-and-a-half 24-hour cycles composed of two 12-hour light periods and three dark periods. VO2, VCO2, heat production and body weights were measured. k, Non-fasting serum insulin was measured by ELISA in indicated male mice (n=8 per group). l, Liver tumor histology in MUP-uPA and DEN- challenged BL6 mice given either CSD, HFrD, or HFrD + Abx cocktail (MUP-uPA n = 7, BL6 n=12 per group). FFPE tumor sections were stained with hematoxylin and eosin (H&E). Representative images are shown. Scale bars, 100 μm. Unpaired two-sided Student’s t test was used in panels c to f and k, whereas two way ANOVA and Sidak’s multiple comparison test was used in panels g-j. Mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.
