Extended Data Fig. 6: Glis1 increased histone lactylation and acetylation at Sall4 and Mycn loci promoting their activation.

(a) Genome views of H3K27Ac tag density at Sall4 and Mycn; red box on the tracks indicates H3K27Ac peaks. Scale bar, 2 kb. For IGV analysis, 1 replicate (n = 1, each group) was used for H3K27Ac analysis in ChIP-seq assay. n = 2 independent experiments were repeated with similar results.(b) ChIP–qPCR analysis of H3K27Ac at Sall4 and Mycn on day 8 in MEFs transduced with SKO plus Flag or Glis1. Data are presented as the mean ± S.D. (n = 3, each group). Group differences are analyzed by the two-tailed Student’s t test. (c) ChIP–qPCR analysis of H3K27Ac at Oct4, Sall4 and Mycn on day 8 in MEFs transduced with SKO plus shLuc or shGlis1. Data are presented as the mean ± S.D. (n = 3, each group). Group differences are analyzed by the two-tailed Student’s t test. (d) ChIP–qPCR analysis of Pan Kla and H3K18la at Oct4, Sall4 and Mycn on day 8 in MEFs transduced with SKO plus shLuc or shGlis1. Data are presented as the mean ± S.D. (Pan kla, n = 3; H3K18la, n=3–4 each group). Group differences are analyzed by the two-tailed Student’s t test.(e) ChIP–qPCR analysis of P300 at Oct4, Sall4, Mycn, Setbp1, Col1a1 and Col5a1 on day 8 in MEFs transduced with Flag or SKO plus Flag. Data are presented as the mean ± S.D. (n = 3, each group). Group differences are analyzed by the two-tailed Student’s t test. (f) Time course of qPCR analysis of endo-Oct4, Sall4, Mycn in MEFs transduced with SKO plus Flag or Glis1. Data are presented as the mean ± S.D. (n = 3, each group). Group differences are analyzed by the two-tailed Student’s t test. (g) The number of peaks defined in each of the OC/CO categories. Two replicate (n = 2, each group) was used for ATAC assay. (h) Time courses of qPCR analyses of T and Eomes in MEFs infected with retroviral SKO plus Flag or Glis1. Data are presented as the mean ± S.D. (n = 3, each group). Group differences are analyzed by the two-tailed Student’s t test.

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