Extended Data Fig. 5: Glis1 enhances reprogramming by glycolysis-driven acetyl-CoA production.
From: Glis1 facilitates induction of pluripotency via an epigenome–metabolome–epigenome signalling cascade
(a) Western blot analysis of H3K27me3, H3K9me3 after Glis1 overexpression or knockdown on day 8 during somatic reprogramming. (n = 1, each group). (b) The effect of acetate on cell proliferation at different concentration. Data are presented as the mean ± S.D. (n = 3, each group). Group differences are analyzed by the two-tailed Student’s t test. (c) The effect of acetate on the level of acetyl-CoA at different concentrations. Data are presented as the mean ± S.D. (n = 3, each group). Group differences are analyzed by the two-tailed Student’s t test. (d) RT-qPCR analysis for detecting the Acly knockdown efficiency in MEFs. (e) OG2 MEFs were infected with retroviral SKO plus shLuc or shAcly addition of acetate. GFP+ colonies were counted on day 20. Data are presented as the mean ± S.D. (n = 3, each group). Group differences are analyzed by the two-tailed Student’s t test. (f) OG2 MEFs were infected with retroviral SKO plus Flag or Glis1 addition of VPA (0.5 mM). GFP+ colonies were counted on day 20. Data are presented as the mean ± S.D. (n = 3, each group). Group differences are analyzed by the two-tailed Student’s t test.(g) OG2 MEFs were infected with retroviral SKO plus shLuc or shGlis1 addition of VPA (0.5 mM). GFP+ colonies were counted on day 20. Data are presented as the mean ± S.D. (n = 3, each group). Group differences are analyzed by the two-tailed Student’s t test. (h) OG2 MEFs were infected with retroviral SKO plus Flag or Glis1 addition of TSA (1 nM). GFP+ colonies were counted on day 20. Data are presented as the mean ± S.D. (n = 3, each group). Group differences are analyzed by the two-tailed Student’s t test. (i) OG2 MEFs were infected with retroviral SKO plus shLuc or shGlis1 addition of TSA (1 nM). GFP+ colonies were counted on day 20. Data are presented as the mean ± S.D. (n = 3, each group). Group differences are analyzed by the two-tailed Student’s t test.
