Extended Data Fig. 6: deoxySA/SA ratios.
From: Serine biosynthesis defect due to haploinsufficiency of PHGDH causes retinal disease
a, DeoxySA/SA ratios following 2, 4, and 8 days of culturing control iPSC-RPE in serine and glycine free media. Each time point run in triplicate. Error bars SEM. b, Relative intracellular deoxySA/SA ratios in WT and PHGDH p.Gly228Trp iPSC-RPE following 2 days in serine and glycine free media. Data represented as mean of nine WT and eight PHGDH p.Gly228Trp replicates from three independently derived clones. c, Relative intracellular deoxySA/SA ratios in control patient and HSAN1 patient iPSC-RPE following 2 days in serine and glycine free media. Data represented as the mean of five independently derived iPSC-RPE clones from two control patients and six independently derived iPSC-RPE clones from two HSAN1 patietns. Error bars SEM. **p<0.01 unpaired two-tailed T-test. b, p=0.0002. c, p=0.0015.
