Extended Data Fig. 10: Activation of Calcr NTS neurons suppresses feeding.
From: A genetic map of the mouse dorsal vagal complex and its role in obesity
a, Representative image showing mCherry immunoreactivity (pseudo-colored green) and FOS immunoreactivity (purple) after CNO treatment in Calcr-Cre mice injected with hM3Dq-mCherry in the NTS (n=7). Scale bar, 150 μm. b,c, Long-term chow food intake (b) and body weight (c) in control (n=6) and Calcr-Cre mice injected with hM3Dq-mCherry in the NTS (n=7) measured during 1 day of saline, 2 days of CNO (i.p., 1 mg kg−1) followed by 1 day of saline treatment. d, Short-term HFD food intake in Calcr-Cre mice injected with hM3Dq-mCherry in the NTS and treated with saline (n=7) or CNO (i.p., 1 mg kg−1; n=7). e,f, Long-term HFD food intake (e) and body weight (f) in control (n=5) or Calcr-Cre mice injected with hM3Dq-mCherry in the NTS (n=7) measured during 3 days of saline, 2 days of CNO (i.p., 1 mg kg-1) followed by 2 days of saline treatment. g, Short-term chow food intake in control mice injected with hM3Dq-mCherry in the NTS and treated with saline (n=6) or CNO (i.p., 1 mg kg-1; n=6). Values are the mean ± s.e.m. P<0.05 are specified, linear mixed effects model, Bonferroni-adjusted least squares means two-tailed t-test. h, Long-term chow food intake in control mice injected with hM3Dq-mCherry in the NTS and treated with saline (n=6) or CNO (i.p., 1 mg kg−1; n=6). Values are the mean ± s.e.m. Linear model, least squares means two-tailed t-test.
