Extended Data Fig. 9: Colchicine-induced GDF15 inhibits pro-IL-1β expression and mature IL-1β secretion from macrophages.
From: Colchicine acts selectively in the liver to induce hepatokines that inhibit myeloid cell activation
Ex vivo activation of immortalized BMDMs evaluated by the levels of (a-b) pro-IL-1β expression and (d-e) IL-1β secretion. a-b, Cells were pre-incubated with plasma from the indicated mice and then activated with Pam3CSK4. Expression of colchicine-induced GDF15 in hepatocytes was required for plasma activity on blocking pro-IL-1β expression. GalNAc-siLuc, hepatocyte-specific siRNA against luciferase. GalNAc-siGDF15, hepatocyte-specific siRNA against GDF15. c, Activation methods for measuring IL-1β production. d, Colchicine did not alter IL-1β production assayed with two activation methods. 5 Samples per condition over 3 biological replicates e, Colchicine-induced GDF15 was required for plasma activity on reducing IL-1β production. 4 Samples per condition over 3 biological replicates Data are represented as mean ± s.d. Two-sided t-tests were used for statistical analysis.
