Extended Data Fig. 3: Depletion of macrophages by clodronate administration.
From: TFEB–GDF15 axis protects against obesity and insulin resistance as a lysosomal stress response
a-e, GTT (a), fasting blood glucose (b), body weight (c), immunohistochemistry of adipose tissue using F4/80 antibody (d), and real-time RT-PCR analysis of F4/80 or Cd11b expression (e) in adipose tissue from HFD-fed TFEBMac-Tg and control mice with or without clodronate treatment (Clod) (n=4 each group). Scale bars, 200 μm. All data are shown as means ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001 by two-way ANOVA with Bonferroni’s test (a) or one-way ANOVA with Tukey’s test (b,c,e). (In a, red asterisk, comparison between untreated TFEBMac-Tg and untreated TFEBMac-Con mice; black asterisk, comparison between treated TFEBMac-Con and untreated TFEBMac-Con mice). (n.s., not significant).
