Extended Data Fig. 1: Tfeb and Gdf15 expression in immune cells and adipose tissue.
From: TFEB–GDF15 axis protects against obesity and insulin resistance as a lysosomal stress response
a, A heatmap showing the relative expression of Tfeb and Gdf15 mRNA in various immune cell types of C57BL/6N mice. b, Tfeb mRNA expression in macrophage and non-macrophage fractions separated by magnetic-activated cell sorting (MACS). c, Tfeb mRNA expression in M1-like and M2-like macrophages estimated by real-time RT-PCR after FACS sorting. d, A heatmap showing relative expression of Tfeb and its target genes in ATM subsets of C57BL/6N mice fed chow diet or HFD (GSE126407). DP-ATM and VA-ATM indicate monocyte-derived CD11c+CD64+ double-positive ATM and vasculature-associated ATM, respectively. VA-ATM1 and VA-ATM2 are distinguished by expression level of MHCII. e, Correlation between VAT TFEB mRNA level and body weight, fasting glucose level or VAT adipocyte size in pooled human subjects (lean, obese and obese T2DM) (n=54). f, Correlation between TFEB mRNA level in SAT and body weight, fasting glucose level or adipocyte sizes of SAT in pooled human subjects (n=54). All data are shown as means ± SEM. *P < 0.05 and **P < 0.01 by one-way ANOVA with Tukey’s test (b,c) or Pearson’s correlation analysis (e,f).
