Extended Data Fig. 4: Gene profiling analyses of iWAT vs. PVAT progenitors and preadipocytes (Related to Fig. 5).
From: Defining the lineage of thermogenic perivascular adipose tissue
a, mRNA levels of indicated genes in differentiated primary adipocytes from: iWAT preadipocytes, aortic PVAT progenitors, aortic PVAT preadipocyte cells (all from P3 CD1 mice). Second experimental replicate performed on different day (n=2 PVAT Pread; n=3 PVAT Progenitor; n=4 iWAT Pread independent wells per group from pooled FACS samples; mean+/- SEM). b, Z-Score split heatmap of genes from overlaps depicted in Fig. 5c,d. Gene expression levels are calculated between cell types (that is PVAT Progenitors vs PVAT PreAd) within a tissue of origin (n=3 biological replicates). c, Expression heatmap of all cell cycle genes (used by Seurat) in sorted progenitor and preadipocyte cells from iWAT and thoracic aortic PVAT. Data comes from DESeq2 normalized count data (bulk RNAseq). d, Pathway analysis of genes enriched in preadipocytes from PVAT vs. iWAT (significantly differentially expressed and LFC>0). Graph plots -log10 unadjusted p values. P values were calculated using clusterProlifer with WikiPathways2019 annotations using the hypergeometric distribution. Padjusted for multiple comparisons was calculated with FDR. Padjusted in order (2.48E-34, 1.07E-20, 1.31E-15, 2.97E-13, 1.37E-12, 2.01E-10, 1.71E-07, 2.66E-07, 1.84E-05).
