Extended Data Fig. 6: Analysis of aortic PVAT in adult mice (Related to Fig. 7).
From: Defining the lineage of thermogenic perivascular adipose tissue
a, Staining for MYH11 (red) and PLIN (green) in aortas of Rosiglitazone (Rosi)-treated mice, scale bar 200 μm. Representative of n=1 experiment. Scale bar, 200 μm. b, mRNA levels of Trpv1 and Gli1 in sorted cell populations. c, Staining of GFP (green) and either PLIN1 or UCP1 (red) in aortas of male Trpv1-Cre; mTmG mice housed at thermoneutrality or following 1 week of 4C cold exposure. Scale bar, 100 μm. d, Staining of TAGLN (red) in adult aorta. White arrowheads show adventitial SMCs. Yellow arrowhead indicates parenchymal SMCs of the aorta. Representative of n=2 experiments. Scale bar, 271.8 μm. e, H&E staining of adult aorta. Arrowheads show adventitial blood vessel. f, Staining of GFP (green) in aortas of Pdgfra-CreER+; mTmG+ mice following a 5-day pulse of Tamoxifen and a 2.5 week treatment with DMSO or Rosi. Arrowheads=GFP+ adipocytes (n=1 Cre-; n=4 DMSO; n=5 Rosi; scale bar, 543.5 μm). g, Staining of GFP (green) in aortas of Pdgfra-CreER+; mTmG+ mice following a 5-day pulse of Tamoxifen and 2-week chase at room temperature or 4C (cold). Representative of n=1 experiment. Scale bar, 100 μm. h, Staining of GFP (green) in aortas of CreER- Ctl and Gli1-CreER+; mTmG+ mice following a 5-day pulse with Tamoxifen and 2.5-week treatment with DMSO or Rosi. (n=1 Cre-; n=3 Cre+; DMSO; n=3 Cre+; Rosi scale bar, 543.5 μm). DAPI (blue) was used to stain nuclei.
