Extended Data Fig. 7: Single nucleus transcriptomic analyses of human PVAT (Related to Fig. 7).
From: Defining the lineage of thermogenic perivascular adipose tissue
a, UMAP of gene expression in 18,758 nuclei from adult human peri-aortic PVAT (Full dataset, n=3 humans, integrated analysis). b, Expression dotplot of indicated genes in dataset from (A). c,d, Violin plots (c) and UMAPs (d) showing expression of select genes, corresponding to the subclustered dataset in Fig. 7d. e, Pathway analysis of cluster defining genes (average logFC>0.5) in SMC and PPARg+ SMC-like cells compared to all other clusters in the dataset from Fig. 7d. Graph plots -log10 unadjusted p values. P values were calculated using clusterProlifer with WikiPathways2019 annotations using the hypergeometric distribution. Padjusted for multiple comparisons was calculated with FDR. Padjusted in order SMC (1.59E-07, 2.50E-06, 2.75E-06, 0.000106107, 0.000968522, 0.000968522, 0.001899498); PPARg+ SMC Like Cells (5.75E-05, 0.000135397, 0.003396457, 0.00971142, 0.00971142, 0.010636022, 0.010636022). f, Violin plots showing expression of select marker genes in the fibroblastic populations from Fig. 7d. g, UMAP of gene expression in 7285 nuclei from adult human deep neck BAT (subclustered to remove immune and endothelial cells, n=16 humans, integrated analysis, re-analyzed data from: Sun et al 40). h-j, UMAPs (h), expression dotplot (i) and violin plots (j) showing expression of select genes in cells from the dataset above (g).
