Extended Data Fig. 5: Investigation of BRD4 and its related histone acetylation markers expression in REDS15 cells upon purine metabolism impairment.
From: Cell-surface SLC nucleoside transporters and purine levels modulate BRD4-dependent chromatin states
a, Western blot analysis of BRD4 expression in chromatin-bound, nucleoplasm and cytoplasmic fractions after 10 μM pelitrexol or lometrexol treatment for 72 h. Lamin B1, GAPDH and histone H3 were markers for nuclear-chromatin, cytoplasm and chromatin-bound protein fractions. b,c, Western blot analysis of BRD4, H3K27ac and pan-H3ac expression in cells with 10 μM 6-MP treatment or bearing triple SLC knockout (SLC TKO, 12 days after sgRNA transduction) (b), and in cells with 10 μM pelitrexol or lometrexol treatment for 72 h (c). ns, non-specific band.
