Extended Data Fig. 7: Transcriptome analysis in cells with purine metabolism impairment.
From: Cell-surface SLC nucleoside transporters and purine levels modulate BRD4-dependent chromatin states
a,b, Heat map of relative transcriptional change (a) and Pearson correlation coefficient of gene expression (b) in REDS1 and REDS15 clones with triple SLC knockout (SLC TKO, 12 days after sgRNA transduction), 10 μM 6-MP or 1 μM (+)-JQ1 treatment for 72 h. The log2 fold change of gene expression was calculated relatively to sgRen or DMSO treated cells. c,d, GSEA of the downregulated genes from the merged RNA-seq results of REDS1 and REDS15 clones (c). The GSEA plots show downregulation of MYC and E2F target gene sets (d). e, RT–qPCR analysis of MYC expression by using two different MYC primer sets in REDS1, REDS15 and K562 cells. Mean ± s.d., n = 3 biologically independent samples. f, Heat map of relative transcriptional change of purine metabolism genes in REDS1 and REDS15 clones with 10 μM 6-MP or 1 μM (+)-JQ1 treatment for 72 h or with triple SLC knockout (SLC TKO, 12 days after sgRNA transduction).
