Extended Data Fig. 4: Identification of the metabolic pathway involving in BRD4 regulation.
From: Cell-surface SLC nucleoside transporters and purine levels modulate BRD4-dependent chromatin states
a, Summary of the targeted pathways of metabolic compound library. b, Representative images of REDS15 cells with DMSO, 1 μM (+)-JQ1, 10 μM CPI-0610 or 10 μM 6-MP treatment for 72 h. Scale bar, 100 μm. c, RFP expression in REDS1 cells with indicated concentrations of 6-MP-related compounds treatment for 72 h. d, RFP expression in REDS1 and REDS15 cells with indicated concentrations of pelitrexol and lometrexol treatment for 72 h. e, RFP expression in REDS1 and REDS15 cells with indicated concentrations of pyrimidine metabolism inhibitors treatment for 72 h. f, Scheme of purine metabolism, enzymes participating in the cascade and the pathway intermediates.
