Extended Data Fig. 1: Pre-operative exercise reduces hepatic I/R injury and inflammation. | Nature Metabolism

Extended Data Fig. 1: Pre-operative exercise reduces hepatic I/R injury and inflammation.

From: Pre-operative exercise therapy triggers anti-inflammatory trained immunity of Kupffer cells through metabolic reprogramming

Extended Data Fig. 1

a, Liver damage were measured by serum AST level (P = 0.7606, P = 0.3610, P = 0.0555, P = 0.0549, P < 0.0001, P < 0.0001, P < 0.0001, P < 0.0001 between exercise and sedentary group from 1day to 16 weeks) and liver histological evaluation (Suzuki scores)) (P = 0.7464, P = 0.1898, P = 0.0932, P = 0.0441, P < 0.0001, P = 0.0002, P < 0.0001, P < 0.0001 between exercise and sedentary group from 1day to 16 weeks) from mice with sedentary or pre-operative exercise treatment for 1 day, or 1 to 16 weeks which subjected to hepatic I/R (n = 6 mice per group). b, Liver damage were measured by plasma ALT (P = 0.7548 in sham group, P < 0.0001 in I/R group) and AST levels (P = 0.2743 in sham group, P < 0.0001 in I/R group) from mice with sedentary or pre-operative exercise treatment for 4 weeks which subjected to hepatic I/R (Each dot represents one mouse, n = 7 mice in Sham group, n = 11 mice in I/R group). c, Liver damage were measured by liver histological evaluation (necrotic areas and Suzuki scores) (for necrotic areas, (P = 0.1228 in sham group, P < 0.0001 in I/R group), for Suzuki scores, (P = 0.8095 in sham group, P = 0.0018 in I/R group) from mice with sedentary or pre-operative exercise treatment for 4 weeks which subjected to hepatic I/R (Each dot represents one mouse, n = 6 mice per group). d, Serum IL-1Ra (P = 0.0001 in 4 weeks group, P = 0.0008 in 8 weeks group, P = 0.0005 in 12 weeks group, P = 0.0006 in 16 weeks group), IL-10 (P = 0.0008 in 4 weeks group, P = 0.0002 in 8 weeks group P = 0.0005 in 12 weeks group P = 0.0002 in 16 weeks group), IL-1β (P = 0.0004 in 4 weeks group, P = 0.0003 in 8 weeks group, P = 0.0002 in 12 weeks group, P = 0.0005 in 16 weeks group), IL-6 (P = 0.0015 in 4 weeks group, P = 0.0011 in 8 weeks group, P = 0.0007 in 12 weeks group, P = 0.0012 in 16 weeks group) and TNF-α (P = 0.0006 in 4 weeks group, P = 0.0011 in 8 weeks group, P = 0.0005 in 12 weeks group, P = 0.0002 in 16 weeks group)concentrations were measured by ELISA from mice with sedentary or pre-operative exercise treatment for 4, 8, 12 or 16 weeks which subjected to hepatic I/R (n = 6 mice per group). e, Serum IFN-α (P = 0.0854 in sham group, P = 0.04854 in I/R group), IFN-β (P = 03053 in sham group, P = 0.1154 in I/R group)and IFN-γ (P = 0.1531 in sham group, P = 0.0015 in I/R group) concentrations were measured by ELISA from mice with sedentary or pre-operative exercise treatment for 4 weeks at baseline (SHAM group) and subjected to hepatic I/R (Each dot represents one mouse, n = 6 mice per group). f, Serum CXCL1 (P < 0.0001 in sham group, P < 0.0001 in I/R group), CXCL2 (P = 0.0040 in sham group, P = 0.0001 in I/R group) and CXCL3 (P = 0.0071 in sham group, P < 0.0001 in I/R group) concentrations were measured by ELISA from mice with sedentary or pre-operative exercise treatment for 4 weeks at baseline (SHAM group) and subjected to hepatic I/R (Each dot represents one mouse, n = 6 mice per group). g, Liver damage were measured by serum AST level (P = 0.0127 between sedentary and 0 day after exercise group, P = 0.0001 between sedentary and 3 days after exercise group, P = 0.0019 between sedentary and 5 days after exercise group, P = 0.0082 between sedentary and 7 days after exercise group, p = 0.4524 between 0 day and 3 days group, P = 0.6955 between 0 day and 5 days group, P = 0.9063 between 0 day and 7 days group, P = 0.6701 between 3 days and 5 days group, P = 0.5088 between 3 days and 7 days group, P = 0.7702 between 5 days and 7 days group) from mice with sedentary or pre-operative exercise treatment for 4 weeks and resting for 0, 3, 5, or 7 days before hepatic I/R (Each dot represents one mouse, n = 11 in sedentary group, n = 7 in 0D after Exercise group, n = 15 in 3D after Exercise group, n = 9 in 5D after Exercise group, n = 7 in 7D after Exercise group). Graphs show the mean ± SD (a to f). Circles, triangles and squares represent individual mice. ANOVA with adjustment for multiple comparisons for (a and d). Unpaired two-sample Student’s t test for (b, c, e and f). NS: not significant. All the statistical test used in this figure was two-sided and two-sided P < 0.05 was considered statistically significant.

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