Extended Data Fig. 4: RT-qPCR validation of RNA sequencing data.
From: Metabolome modulation of the host adaptive immunity in human malaria
a, Box plots show matched relative expression of levels of 19 T cell transcription factors, ligands and receptors before (blue) and during (red) infection quantified using RNASeq (n = 36 children) and RT-qPCR (n = 23 children). Bar and whiskers represent mean ± SD. Differences in RNASeq and qPCR data were assessed using paired two-tailed Student’s t-test and Wilcoxon test, respectively (****P <0.0001). b, Bar plot shows the log2 fold change of expression levels for each of the 19 genes between before and during infection stages using RNASeq (blue) and RT-qPCR (orange) data. c, Correlation plots showing the moderation effect of infection on the association between pregnenolone sulfate and lymphocytes percentage (left panel) through the expression of CD274 (right panel) quantified in 23 individuals before infection (blue) and during infection (red) using RT-qPCR.
