Extended Data Fig. 1: Hepcidin expression is increased across many cancer types.
a, Hepcidin transcript abundance in normal and tumor tissue through The Cancer Genome Atlas. Log2 median centered ratio is plotted (Bladder Normal N = 24 and Bladder Tumor N = 152; Brain Normal N = 10 and Brain Tumor N = 547; Colorectal Normal N = 22 and Colorectal Tumor N = 215; Breast Normal N = 61 and Brest Tumor N = 532; Cervical Normal N = 5 and Cervical Tumor N = 155; Head/Neck Normal N = 74 and Head/Neck Tumor N = 388; Kidney Normal N = 329 and Kidney Tumor N = 727; Leukemia Normal N = 195 and Leukemia Tumor N = 197; Liver Normal N = 30 Liver Tumor N = 138; Lung Normal N = 71 and Lung Tumor N = 984, Lymphoma Normal N = 18 and Lymphoma Tumor N = 18, Ovary Normal N = 88 and Ovary Tumor N = 607; Pancreas Normal N = 10 and Pancreas Tumor N = 64; and Prostate Normal N = 34 and Prostate Tumor N = 171 biologically independent samples, Brain p = 0.0047. Breast p = 0.0009. Colorectal p = 0.0006. Kidney p < 0.0001, Lung p = 0.0092. Ovary p = 0.0002. Prostate p = 0.0222). b, qPCR analysis of hepcidin (Hamp) expression levels in tissues/cells of wild-type and colon epithelial-specific APC deficient mice (WT N = 4 and APC-/- N = 4 biologically independent samples from independent animals in each tissue). c, Hepcidin concentration in serum of wild-type and colon epithelial-specific APC deficient mice (WT N = 4 and APC-/- N = 4 biologically independent samples from independent animals). Data represent the mean ± SEM. Significance was determined by 2-tailed, unpaired t test. a-c, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 compared to normal tissue or wild-type animals.
