Extended Data Fig. 5: Acs2 and Sas2 are required for acetate to induce H4K16ac.
From: The SESAME complex regulates cell senescence through the generation of acetyl-CoA
a Acetate has no significant effect on Acs2 protein levels as determined by western blots. WT (BY4741) cells were treated with 0–50 mM KAc for 4 hr. KCl was added to keep the concentration of K+ at 50 mM. b Effect of acetate on H4K16ac in WT TetO7 and TetO7-ACS2 mutants as determined by Western blots. WT TetO7 and TetO7-ACS2 mutant were treated with KAc and doxycycline (dox) for 0–5 hr. c Effect of acetate on H4K16ac in WT and sam1Δ mutant. d and e Effect of acetate on H4K16ac in acs1Δ (d) and ach1Δ (e) mutants. f Acetate has no significant effect on Sas2 occupancy at subtelomere regions as determined by ChIP-qPCR. g Acetate treatment had no effect on the interaction between Sas2 and Sas5 as determined by Co-IP assay. The Sas5-13Myc/Sas2-FLAG and Sas5-13Myc/Sas2-FLAG/acs2-ts cells were grown at 37 °C and treated with or without KAc for 4 hr. Sas5 (Sas5-13Myc) was immunoprecipitated with anti-Myc agarose from cell lysates. The co-IPed Sas2 was detected by anti-FLAG (Sas2-FLAG). For Extended Data Fig. 5a–f, data represent means ± SE; n = 3 biological independent experiments. Two-sided t-tests were used for statistical analysis. For Extended Data Fig. 5g, shown is the typical example of 2 biological independent replicates.
