Extended Data Fig. 6: Acetate regulates telomere silencing in budding yeast.
From: The SESAME complex regulates cell senescence through the generation of acetyl-CoA
a Histogram showing the fraction of genes down-regulated in bdf1Δ (≤0.75-fold, P < 0.05) when plotted as a function of their distance to the nearest telomere. Genes were categorized at 10-kb intervals for up to 60 kb from telomeres. One-sided χ2 tests were used. *, χ2 > 3.841, P < 0.05; ***, χ2 > 10.828, P < 0.001. b Effect of Acs2 on Bdf1 expression as determined by western blots. c Effect of Bdf1 on the global levels of H4K16ac as determined by western blots. d ChIP analysis of H4K16ac at TEL V and TEL VII regions in WT and bdf1Δ mutant. e Effect of acetate on Bdf1 occupancy at subtelomere regions (TEL V XC, TEL V XR, TEL VII XC and TEL VII XR) as determined by ChIP-qPCR analysis. f ChIP-qPCR analysis of Sir2 occupancy at regions with different distance to TEL VI-R in TEFpr-ACS2 and CYCpr-ACS2. g Effect of Acs2 on Sir2 protein levels as determined by western blots. WT and acs2-ts mutant were grown at 37 °C for 2 hr. h ChIP-qPCR analysis of the effect of acetate on Sir2 binding at telomere-proximal genes in acs2-ts mutant. KCl was added as a control. i and j RT–qPCR analysis of the effect of KAc and sodium acetate (NaAc) on the transcription of telomere-proximal genes. KCl and NaCl were used as controls. k and l qRT-PCR analysis of the effect of acetate on the transcription of telomere-proximal genes in sam1Δ (k) and acs2-ts (l) mutants. m qRT-PCR analysis of the transcription of telomere-proximal genes in WT cells treated with YP + 2% glucose, 0.5% glucose, 0.05% glucose, 2% glycerol, 2% ethanol. For Extended Data Fig. 6b–m, data represent means ± SE; n = 3 biological independent experiments, two-sided t-tests were used for statistical analysis.
