Extended Data Fig. 8: Acetate accelerates cell senescence in HUVEC.
From: The SESAME complex regulates cell senescence through the generation of acetyl-CoA
a Effect of acetate on HUVEC cell growth. b Effect of acetate on HUVEC cell cycle profiling. c-e Quantification of western blots data in Fig. 8a,b,e. f Effect of glucose (Gluc) starvation on H4K16ac in control (siControl) and ACSS2 knockdown (siACSS2) HUVEC cells. Cells were depleted of glucose for 6 hr. g Effect of glucose (Gluc) starvation on senescence of control (siControl) and ACSS2 knockdown (siACSS2) HUVEC cells as determined by SA-β-gal staining. Scale bar, 25 μm. Right panel: Quantification of the percentage of SA-β-gal positive cells. h Effect of glucose starvation on transcription of IL1A, IL1B, p16 and p21 in control (siControl) and ACSS2 knockdown (siACSS2) HUVEC cells. For Extended Data Fig. 8a–h, the quantitative data represent the mean ± SE; n = 3 biological independent experiments; two-sided t-tests were used for statistical analysis.
