Extended Data Fig. 8: Absolute quantification of NAD+ and its precursors (NAM, NR and NMN) in the brains, primary neurons, and culture media after NAD+ or NAM supplementation.
From: Aberrant NAD+ metabolism underlies Zika virus–induced microcephaly
a, NAD+ concentrations in the homogenous extract of cortex and hippocampus of ZIKV-infected mouse at indicated time points post vehicle (RPMI medium 1640 basic + 2% FBS) or NAD+ (500 μM, 2 μL) injection into the λ point (n = 4 mice for 0 h, 1 h, 2 h, 12 h time points, n = 3 mice for 6 h time point). b, NAD+ and NAM concentrations in the homogenous extract of cortex and hippocampus of ZIKV-infected mouse at indicated time points post vehicle (RPMI medium 1640 basic + 2% FBS) or NAM (500 μM, 2 μL) injection into the λ point (n = 5 mice for 0 h, 2 h, 6 h, 12 h time points; n = 4 mice for 1 h time point). c, Concentrations of NAD+ and its precursors in primary neurons before (zero time point) and after being cultured with 1 mM NAD+ for 0.5 h to 12 h (n = 5 biological replicates per time point). d, Concentrations of NAD+ and its precursors in culture media at indicated time points (n = 5 biological replicates per time point). Fresh media at zero time point contains 1 mM NAD+. Data are shown as mean ± s.e.m. (a-d). Statistical analysis was performed using One-way ANOVA followed by Benjamini and Hochberg multiple comparisons (a-d). Each time point was compared with zero time point. Exact P values are indicated.
