Extended Data Fig. 4: The Role of DMiro in Lifespan.
For all panels, ‘RU-‘ flies were fed with the same volume of the vehicle, ethanol. All flies were female. (a) Survival curves of wild-type flies (w1118) fed with or without 200 μM RU. n=141 flies. p=0.46 by Log-Rank Test. (b) Later life-onset neuronal downregulation of DMiro using Elav-GS-GAL4 induced in the presence of RU from day 30 (Elav-GS>UAS-DMiroRNAi, RU+, blue line) extends lifespan, compared to un-induced controls (RU-, black line). n=106 (RU+) and 127 (RU-) flies. Median lifespan was increased by 10%, p=1.3×10-7 by Log-Rank Test. (c) Adult-onset downregulation of DMiro using the intestine enterocytes driver 5966-GS-GAL4 induced in the presence of RU (5966-GS>UAS-DMiroRNAi, RU+, red line) shortens lifespan, compared to un-induced controls (RU-, black line). n=142 (RU+) and 151 (RU-) flies. p=0.0007 by Log-Rank Test. (d) Later life-onset downregulation of DMiro in intestine enterocytes using 5966-GS-GAL4 induced in the presence of RU from day 30 (5966-GS>UAS-DMiroRNAi, RU+, red line) slightly extends lifespan, compared to un-induced controls (RU-, black line). n=135 (RU-) and 145 (RU+) flies. p=0.03 by Log-Rank Test. (e) Adult-onset ubiquitous knockdown of DMiro using Da-GS-GAL4 in the presence of RU (Da-GS>UAS-DMiroRNAi, RU+, red line) shortens lifespan, compared to un-induced controls (RU-, black line). n=143 (RU+) and 144 (RU-) flies. p=2.07×10-9 by Log-Rank Test. (f) Later life-onset ubiquitous knockdown of DMiro using Da-GS-GAL4 in the presence of RU from day 30 (Da-GS>UAS-DMiroRNAi, RU+, red line) does not alter lifespan, compared to un-induced controls (RU-, black line). n=141 (RU-) and 146 (RU+) flies. p=0.74 by Log-Rank Test. (g) Adult-onset downregulation of DMiro using the muscle driver MHC-GS-GAL4 induced in the presence of RU (MHC-GS>UAS-DMiroRNAi, RU+, red line) shortens lifespan, compared to un-induced controls (RU-, black line). n=146 (RU-) and 149 (RU+) flies. p=1.68×10-16 by Log-Rank Test. (h) Adult-onset neuronal downregulation of white using Elav-GS-GAL4 induced in the presence of RU (Elav-GS>UAS-whiteRNAi, RU+, red line) slightly shortens lifespan, compared to un-induced controls (RU-, black line). n=139 flies. p=0.01 by Log-Rank Test.
