Extended Data Fig. 6: The Role of MICOS in Lifespan.
(a) Later life-onset downregulation of dMIC19 using the intestine enterocytes driver 5966-GS-GAL4 induced in the presence of RU from day 30 (5966-GS>UAS-dMIC19 RNAi, RU+, red line) does not alter lifespan, compared to un-induced controls (RU-, black line). n=143 (RU+) and 146 (RU-) flies. p=0.84 by Log-Rank Test. (b) Adult-onset downregulation of dMIC19 in intestine enterocytes induced in the presence of RU (5966-GS>UAS-dMIC19 RNAi, RU+, red line) does not alter lifespan, compared to un-induced controls (RU-, black line). n=144 (RU+) and 150 (RU-) flies. p=0.59 by Log-Rank Test. (c) Later life-onset downregulation of dMIC60 in intestine enterocytes induced in the presence of RU from day 30 (5966-GS>UAS-dMIC60 RNAi, RU+, red line) does not alter lifespan, compared to un-induced controls (RU-, black line). n=142 (RU+) and 143 (RU-) flies. p=0.99 by Log-Rank Test. (d) Adult-onset downregulation of dMIC60 in intestine enterocytes induced in the presence of RU (5966-GS>UAS-dMIC60 RNAi, RU+, red line) does not alter lifespan, compared to un-induced controls (RU-, black line). n=140 (RU+) and 147 (RU-) flies. p=0.94 by Log-Rank Test. (e) The survival ability of female flies with a second DMiro RNAi line driven by Elav-GS-GAL4 induced by RU since adulthood (Elav-GS>UAS-DMiroRNAi 2, blue line, RU+) or of un-induced control flies (black line, RU-), in response to 20 mM paraquat (starting from day 7). n=14 (RU+) and 56 (RU-) flies. p=0.016 by Log-Rank Test. (f) Fly heads were lysed and blotted as indicated. The band intensity is normalized to that of α-tubulin from the same blot. n=4. One-Way Anova Post Hoc Tukey Test (p=0.6915 for ubiquitin, p=0.5884 for p62). Boxes show 25th/75th percentiles, whiskers are the minimum and maximum, and middle line is median.
