Extended Data Fig. 3: Effect of hepatocyte-specific Rab27 knockout on metabolic phenotypes.
From: Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
a, Hepatocyte Rab27 levels in the hepatocyte-specific Cas9 transgenic mice after 4 weeks of injection of lentivirus harboring gRNA Rab27 vectors. Control mice were treated with a lentiviral empty vector. b, Effect of 4wk HFD Rab27KO hepatocyte Exos on glucose production in 16wk HFD/obese primary hepatocytes. Empty liposomes were used in the control cells. c, Body weight of both WT and hepatocyte-specific Rab27KO mice after 4 weeks HFD feeding. d, Hepatic glucose production after Rab27 knockout. All experiments were repeated at least twice with similar results. Data are presented as mean ± SEM. n=4 (b), n=6 (c), n=8 (d) per group. Two-sided Student’s t-test.
