Extended Data Fig. 4: Effect of miR-3075 on insulin sensitivity.
From: Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
miR-3075 abundance in liver (a), skeletal muscle (SKL, b), and eWAT (c) of 18wk HFD WT recipient mice after treatment with 4wk HFD Exos. qPCR analysis of miR-3075 abundance (d), hepatic glucose production (e), and insulin-stimulated AKT phosphorylation (f) in lean, 4wk or 16wk HFD WT hepatocytes. g, Effect of the miR-3075 antagomir on AKT phosphorylation level in 4wk HFD hepatocytes. h, miR-3075 abundance in key metabolic tissues of lean WT mice, 3T3-L1 adipocytes, and L6 myocytes. i, Effect of 4wk HFD Exos, 4wk HFD YBX1KO Exos, or combination treatment with 4wk HFD YBX1KO plus miR-3075 mimic on cellular insulin responses. j, Glucose tolerance of NCD, 4wk HFD, and 16wk HFD WT mice. k, qPCR analysis of miR-3075 abundance within Exos derived from lean hepatocytes transfected with the miR-3075 mimic. l, Effect of miR-3075-enriched hepatocyte (miR-3075oe) Exos on 3T3-L1 adipocyte glucose uptake. m, The levels of phosphorylated AKT in metabolic tissues of 4wks HFD WT mice after treatment with the miR-3075 antagomir. Effects of miR-3075 antagomir on glucose tolerance (n) and insulin sensitivity (o) of lean WT mice. n=4 (a-c, h, k), n=6-8 (d), n=8 (e), n=6 (i), n=8 (j, l), n=5-6 (n and o) per group. All experiments were repeated at least twice with similar results. Data are presented as mean ± SEM. Two-sided Student’s t-test.
