Extended Data Fig. 2: Effect of hepatocyte-specific YBX1 knockout on the ability of hepatocyte Exos to regulate metabolic responses.
From: Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
a, Hepatocyte YBX1 levels in the hepatocyte-specific Cas9 transgenic mice after 4 weeks of injection of lentivirus harbouring gRNA-YBX1 vectors. Control mice were treated with a lentiviral empty vector. b, Expression of TSG101 and CD63 in Exos derived from both 4wk HFD WT and YBX1KO hepatocytes. c, Body weight of both WT and YBX1KO mice after 4 weeks HFD feeding. d, Effect of hepatocyte Exos on body weight of 18wks HFD/obese recipient mice. Empty liposomes were used in the control HFD WT mice. e, Effect of 4wk HFD WT Exos or YBX1KO Exos on cellular phosphorylated AKT levels. Control cells were treated with empty liposomes. All experiments were repeated at least twice with similar results. Data are presented as mean ± SEM. n=8 (c and d) per group.
