Extended Data Fig. 5: Regional identity of villi enterocytes is changed upon HFHSD.
From: Diet-induced alteration of intestinal stem cell function underlies obesity and prediabetes in mice
a, Overview of the experimental setup for scRNA-seq of villi cells. b, UMAP plot of 4205 profiled single SI villi cells. Colors highlight clustering into major intestinal cell types based on expression of previously published marker genes17. c, UMAP plot of enterocyte progenitors (EP) and enterocytes computed using a set of 44 transcription factors previously described to be differential between proximal and distal gut regions17. Colors highlight diet. d, UMAP plot of EP and enterocytes computed using a set of 44 transcription factors previously described to be differential between proximal and distal gut regions17. Colors highlight regional annotation. e, Violin plots showing cell scores of the proximal and distal transcription factors used in c)17 in CD and HFHSD-derived EPs and enterocytes. f, Proportions of enterocytes and EPs annotated as proximal- or distal-type enterocytes based on regional transcription factor scores in c. g, Heatmap showing the log2- fold change of the expression of selected functional proximal genes in HFHSD vs CD distal and proximal enterocyte cluster. *indicates a significant change. The p-values are provided in Supplementary table 1.
