Extended Data Fig. 6: Enterocyte zonation is altered upon HFHSD.
From: Diet-induced alteration of intestinal stem cell function underlies obesity and prediabetes in mice
a, Activation of zonation scores along a pseudospatial ordering of EP and enterocytes from SI villi, which reflects the axis from villus bottom to tip. Cell scores for each zone were computed on reported markers18 and approximated by polynominal regression fits along the pseudospatial axis. Crossing points of the fitted lines define the partitioning into the five zones. b, Dot plots showing the expression of selected markers of enterocyte function in distal and proximal enterocytes. Cells are partitioned into zones along the inferred pseudospatial axis from villus bottom (zone 1) to tip (zone 5). c–e, Representative LSM images (c) and quantification of Fabp1 mean fluorescence intensity in ileal villi (d) and determination of the length of the Fabp1 positive zone (e). Scale bar, 50 µm, n = 3 CD mice and n = 4 HFHSD mice. Data are shown as mean ± s.d. of biologically independent samples. Statistical significance was determined by two-tailed Student’s t-test. f–h, Representative LSM images (f) and quantification of Apoa4 mean fluorescence intensity in ileal villi (g) and determination of the length of the Apoa4 positive zone (h). Scale bar, 50 µm, n = 3 CD mice and n = 4 HFHSD mice. Data are shown as mean ± s.d. of biologically independent samples. Statistical significance was determined by two-tailed Student’s t-test. i–k, Representative LSM images (i) and quantification of Apoa4 mean fluorescence intensity in duodenal villi (j) and determination of the length of the Apoa4 positive zone (k). Scale bar, 50 µm, n = 5 mice per group. Data are shown as mean ± s.e.m. of biologically independent samples. Statistical significance was determined by two-tailed Student’s t-test.
