Extended Data Fig. 8: HFHSD increases turnover of SI crypt cells.
From: Diet-induced alteration of intestinal stem cell function underlies obesity and prediabetes in mice
a, Left: Cell score levels for cell-cycle phases S and G2/M calculated based on the expression of a set of genes related to cell-cycle are visualized in a UMAP plot. Right: Cells assigned to the corresponding cell-cycle phase are highlighted in red. b, Proportions of cycling cells assigned to S and G2/M cell-cycle phases in CD and HFHSD-derived in indicated clusters. Table indicates percentages. Data are shown as mean ± s.e.m., n = 3 mice per group. c, Assessment of body weight over the course of 14 weeks of CD- and HFHSD-fed Lgr5-ki mice. n = 4 per group. Data are shown as mean ± s.e.m. of biologically independent samples. Statistical significance was determined by two-tailed Student’s t-test. d, Representative FACS plots showing gating strategy to determine the proportion of Lgr5-EGFPhi cells (ISCs) in CD and HFHSD fed Lgr5-ki mice. e, f, Representative LSM images (e) and quantification (f) of Ki67+ (green) proliferative regions. Scale bar, 75 µm, n = 4 mice per group. Data are mean ± s.e.m. of biologically independent samples. Statistical significance was determined by two-tailed Student’s t-test. g–i, Representative LSM images of BrdU-labelled cells (BrdU+ nuclei, red) along the crypt-villus axis 24 h after BrdU administration in duodenal sections from CD- and HFHSD-fed FVF mice (g). Quantification of cell migration length by measuring the distance from the crypt base to the highest labelled cell in the villus (h). Net BrdU migration (i), calculated as the average distance of BrdU-labelled migration front minus the Ki67+ zone to correct for increased cell proliferation on HFHSD. Scale bar, 25 µm. n = 4 mice per group. Data are mean ± s.e.m. of biologically independent samples. Statistical significance was determined by two-tailed Student’s t-test.
