Extended Data Fig. 4: Analysis of Foxa2 lineage positive cells and frequency of mature intestinal cell types in FVF mice in CD and HFHSD conditions.

a, Representative LSM images of tamoxifen-induced recombination in duodenal crypts of CD- and HFHSD-fed Foxa2^{nEGFP-CreERT2/+}; Gt(Rosa26)^mTmG/+ mice after 48 h. Yellow arrows indicate recombined mG⁺ slender crypt base cells (CBC) at positions typical for ISCs, adjacent to pyramid-shaped Paneth cells. Scale bar, 25 µm. n = 3 biologically independent CD and HFHSD samples. b, Frequencies of coherent Foxa2-lineage ribbons of different sizes in the villi of CD- and HFHSD-fed Foxa2^{nEGFP-CreERT2/+}; Gt(Rosa26)^mTmG/+ mice at 70 h after tamoxifen induction. n = 3 mice per group. Data are mean ± s.e.m. of biologically independent samples. Statistical significance was determined by two-tailed Student’s t-test. c, Percentage of villin⁺ enterocytes, Muc2⁺ goblet cells and ChgA⁺ EECs within Foxa2 lineage ribbons. n = 3 mice per group. Data are mean ± s.e.m. of biologically independent samples. Statistical significance was determined by two-tailed Student’s t-test. d, e, Quantification of ChgA⁺ EECs (d, n = 3 mice per group) and Muc2⁺ goblet cells (e, n = 4 mice per group) in ileal tissue sections of CD- and HFHSD-fed FVF mice. Data are shown as mean ± s.e.m. of biologically independent samples. Statistical significance was determined by two-tailed Student’s t-test. f, g, Representative LSM images (f) and quantification (g) of Paneth cells (Lyz1⁺) in the duodenum. Scale bar, 25 µm, n = 8 mice per group. Data are mean ± s.e.m. of biologically independent samples.

Source data