Extended Data Fig. 6: The effect of three calorie restriction regimens on female C57BL/6J mice.
(a) Outline of feeding regimens: AL, Diluted AL, CR and MF.cr. (b-e) Body composition measurement over 16 weeks on diet (AL, n = 12; Diluted AL, n = 10; MF.cr, n = 12; CR, n = 12 biologically independent mice); total body weight (b), lean mass (c), fat mass (d) and adiposity (e). (f-g) Glucose (n = 12 biologically independent mice per diet) (f) and insulin (AL, n = 12; Diluted AL, n = 12; MF.cr, n = 11; CR, n = 10 biologically independent mice) (g) tolerance tests after 9 or 10 weeks on the indicated diets. * symbol represents a significant difference versus AL-fed mice (Diluted AL, p < 0.0001; MF.cr, p ≤ 0.0012, CR, p < 0.0001); # symbol represents a significant difference versus Diluted AL-fed mice (MF.cr, p = 0.0019; CR, p ≤ 0.0001); @ symbol represents a significant difference versus MF.cr-fed mice (CR, p = 0.0043) based on Tukey’s test post one-way ANOVA. (h-j) Metabolic chamber analysis of mice fed the indicated diets. (h) Respiratory exchange ratio vs. time (n = 12 biologically independent mice per diet) (i) Fuel utilization was calculated for the 24-hour period following the indicated (arrow) refeeding time (n = 12 biologically independent mice per diet). * symbol represents a significant difference versus AL (Diluted AL, p = 0.0255); # symbol represents a significant difference versus Diluted AL (CR, p = 0.0274) based on Tukey’s test post one-way ANOVA performed separately for FAO and C/PO). (j) Energy expenditure as a function of lean mass was calculated for the 24-hour period following the indicated (arrow) refeeding time (n = 12 biologically independent mice per diet, data for each individual mouse is plotted; slopes and intercepts were calculated using ANCOVA). k) Food consumption (AL, n = 12; Diluted AL, n = 12; MF.cr, n = 12; CR, n = 11-12). All data are represented as mean ± SEM.
