Extended Data Fig. 2: Control of hypothalamic nuclei dissections.

Quantification of Pomc, Sf1 and Hcrt/orexin mRNA levels in ARC, VMH and LHA dissections [Pomc: ARC n = 20 mice, VMH n = 20 mice, LHA n = 19 mice; Sf1: ARC n = 19 mice, VMH n = 19 mice, LHA n = 19 mice; Hcrt: ARC n = 20 mice, VMH n = 20 mice, LHA n = 19 mice; box plots indicate median (middle line), 25th, 75th percentile (box) and 10th-90th percentiles (whiskers; minima and maxima, respectively)]. Data expressed as mean ± SEM. ***P < 0.001 vs. Pomc ARC, Sf1 VMH and Hcrt LHA. Statistical significance was assessed by two-sided ANOVA.