Extended Data Fig. 4: Effect of systemic treatment with SF1-AMPKα1-DN loaded sEVs on central and peripheral tissues in DIO mice.
a, Quantification of pACCα/ACCα levels in cortex, thalamus and cerebellum after 28-day of intravenous injection with control (non-loaded; cortex n = 7 mice; thalamus n = 7 mice; cerebellum n = 6 mice) or SF1-AMPKα1-DN loaded (cortex n = 7 mice; thalamus n = 7 mice; cerebellum n = 7 mice) sEVs. b, Quantification of pACCα/ACCα levels in liver, adrenal gland, testis, BAT, heart and skeletal muscle after 28-day of intravenous injection with control (non-loaded, liver n = 7 mice; adrenal n = 7 mice; testis n = 6 mice; BAT n = 7 mice; heart n = 7 mice; skeletal muscle n = 6 mice) or SF1-AMPKα1-DN loaded (liver n = 7 mice; adrenal n = 7 mice; testis n = 7 mice; BAT n = 7 mice; heart n = 7 mice; skeletal muscle n = 7 mice) sEVs. c, Quantification of pACCα/ACCα levels in brown adipocytes after 24 h of incubation with control (non-loaded) or SF1-AMPKα1-DN loaded (n = 5 samples/group) sEVs. d, Quantification of circulating testosterone levels after 28-day of intravenous injection with control (non-loaded) or SF1-AMPKα1-DN loaded (n = 7 mice/group) sEVs. e, Quantification of mRNA levels of steroidogenic enzymes (STAR, p450scc and 17β-HSD3) in testis after 28-day of intravenous injection with control (non-loaded) or SF1-AMPKα1-DN loaded (n = 7 mice/group) sEVs. f, Quantification of circulating CORT levels after 28-day of intravenous injection with control (non-loaded, n = 7 mice) or SF1-AMPKα1-DN loaded (n = 6 mice) sEVs. g, Quantification of mRNA levels of P450scc and STAR in adrenals after 28-day of intravenous injection with control (non-loaded; P450scc n = 5 mice; STAR n = 6 mice) or SF1-AMPKα1-DN loaded (P450scc n = 5 mice/group; STAR n = 5 mice/group) sEVs. h, Quantification of circulating LH levels after 28-day of intravenous injection with control (non-loaded) or SF1-AMPKα1-DN loaded (n = 6 mice/group) sEVs. i, Quantification of mRNA levels of LH β subunit in pituitary after 28-day of intravenous injection with control (non-loaded, n = 6 mice) or SF1-AMPKα1-DN loaded (n = 7 mice) sEVs. a-i, j, Quantification of BAT UCP1 levels at 1, 2, 3 and 7 days after a single injection in the tail vein of control (non-loaded; day 1 n = 6 mice; day 2 n = 5 mice; day 3 n = 5 mice; day 7 n = 5 mice) or SF1-AMPKα1-DN sEVs (day 1 n = 4 mice; day 2 n = 4 mice; day 3 n = 5 mice; day 7 n = 5 mice) at day 0; day 1 P = 0.023; day 2 P = 0.0054. Data expressed as mean ± SEM. *P < 0.05 and **P < 0.01 vs. Control. Statistical significance was assessed by two-sided Student’s t-test.
