Fig. 6: ATGL levels are regulated by ROS in HepG2 cells and repressed by overloaded FAs.
From: Peroxisomal β-oxidation acts as a sensor for intracellular fatty acids and regulates lipolysis
a,b, HepG2 cells were transfected with CAT or ACOX1 siRNA. After 48 h, IB was conducted to analyse protein levels using the indicated antibodies (n = 6 in CAT and 12 in Nc siRNA, F = 30.1 (a); n = 5 in ACOX1 and 8 in Nc siRNA, F = 20.62 (b)). c,d, HepG2 cells were treated with H2O2 or NAC and collected at the indicated time points. IB was conducted to analyse protein levels using the indicated antibodies (n = 4 in H2O2 treatment and 9 in control, F = 9.689 (c); n = 5 in 6 mM and 7 mM NAC, 6 in 5 mM and 8 mM NAC and 9 in control, F = 47.12 (d)). e, iBAs with PEX2–FLAG–EGFP expression were treated with 0.1 μM Iso and collected at the indicated time points. IP and IB were conducted using the indicated antibodies (n = 4, F = 11.01). f, iBAs were transfected with Nc and Pex2 siRNA. After 48 h, cells were stimulated with 0.1 μM Iso for 24 h at the indicated doses. IB was conducted to check protein levels using the indicated antibodies (n = 4). g–i, iBAs were treated with 100 μM of various FAs. After 48 h, cells were collected for IB analysis or lipolysis measurement was performed (n = 5, F = 14.35 (g), 16.51 (h) and 7.892 (i)). Results are shown as the mean ± s.e.m. and analysed using a two-sided Student’s t-test (f) or ANOVA with Dunnett correction for multiple comparisons between control and other groups (a–e,g–i).
