Extended Data Fig. 4: Analysis of master gene regulators in differentiating Opa1tg preadipocytes.
(a) Heat map of the expression levels of the Opa1-specific downregulated genes subset identified in Fig.4h. Each column corresponds to the indicated sample. The dendrogram clustering on the Y-axis groups genes with similar expression profiles. Gene expression levels are indicated per each row. (b) Upstream regulators for the DEGs dataset in Fig.4h by Upstream Regulator Analysis in IPA. y-axis corresponds to the Log10 (p-Value), and the x-axis displays the z-score activation values. (c) Expression fold change of the indicated genes in Opa1tg pre-adipocytes transduced with shRNAs against Hif1α (shHif1α) or an untargeted control sequence (shUT) and differentiated into brite adipocytes (n = 10 independent experiments). Dots indicate individual experiments, center lines the mean, whiskers SEM. *, p = 0.001 for Hif1α and 0.003 for Ucp1 in a two-tailed Mann-Whitney U test. (d) Selected DEGs identified in Fig. 4i were used to identify functional relationships among selected genes by IPA. (e) Kdm3a expression fold change (brite vs. preadipocytes). Pre-adipocytes of the indicated genotype transduced with the indicated shRNA were differentiated into brite adipocytes. N = 3 independent experiments from 6 pooled Opa1tg or Wt mice/experiment. Dots indicate individual experiments, box represents mean ± SEM, whiskers the 10th-90th percentile.; *p = 0.04, **p = 0.01 in two-tailed sample t-test.
