Extended Data Fig. 6: Mass isotopomer distribution in flux analysis experiments. | Nature Metabolism

Extended Data Fig. 6: Mass isotopomer distribution in flux analysis experiments.

From: The mitochondrial protein Opa1 promotes adipocyte browning that is dependent on urea cycle metabolites

Extended Data Fig. 6

(a) Mass isotopomer distribution (MID) of fumarate and aspartate in Opa1tg and Wt preadipocytes following [13C3]-pyruvate infusion. (b) Mass isotopomer distribution of IMP, Aspartate, Fumarate, Arginine, Glutamate in Opa1tg and Wt preadipocytes following [13C4,15N]-L-aspartic acid infusion. (c) Mass isotopomer distribution of fumarate and aspartate in Opa1tg preadipocytes transduced with control (shScr) shRNA and against Cps1 (shCps1) following [13C3]-pyruvate infusion. (d) Mass isotopomer distribution of IMP, Aspartate, Fumarate, Arginine, Glutamate in Opa1tg preadipocytes transduced with control (shScr) shRNA and against Cps1 (shCps1) following [13C4,15N]-L-aspartic acid infusion. In all graphs, unlabeled (m + 0) and labeled isotopomers are shown. Bars represents the average value from 2 indicated (dots) independent measurements (n = 4 pooled mice/genotype/experiment).

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