Extended Data Fig. 7: Adipocyte Opa1 deletion causes lipoatrophy and metabolic dysfunction.
(a) Equal amounts of protein (20 µg) from brown adipose tissue (BAT), subcutaneous (SAT) and visceral (VAT) white adipose tissues, heart and liver lysates from mice of the indicated genotypes were separated by SDS-PAGE and immunoblotted using the indicated antibodies (n = 7/genotype). (b) Representative photographs of SAT and VAT from control and Opa1ΔAT mice (n = 7/genotype). Scale bar: 1 cm. (c) Box-dot plots of SAT and VAT weights from control and Opa1ΔAT mice (n = 7-8). **, p = 0.009 Opa1ΔAT vs. Wt SAT; p = 0.001 Opa1ΔAT vs. Wt VAT in a two-tailed Mann-Whitney U test. (d) Body composition assessed by EchoMRI in Wt (n = 23) and Opa1ΔAT (n = 20) mice. *, p = 0.04 Opa1ΔAT vs. Wt lean mass; ***, p = 2.2×10−8 Opa1ΔAT vs. Wt fat mass in a two-tailed Mann-Whitney U test. (e) Representative Hematoxylin-Eosin staining of SAT and VAT from control and Opa1ΔAT mice (n = 7/genotype). (f) Box-dot plots of adipocytes area in control and Opa1ΔAT mice. (n > 1000 adipocyte/mouse; 4 mice/genotype). ***, p = 1×10−5 in a two-tailed Mann-Whitney U test between groups. (g,h) Box-dot plots of Serum Adiponectin (g) and Insulin (h) levels measured by ELISA in control and Opa1ΔAT mice (n = 5-8). **, p = 0.01 for Adiponectin and p = 0.004 for Insulin in a two-tailed Mann-Whitney U test. (i) Representative photographs of livers from control and Opa1ΔAT mice (n = 7/genotype). Scale bar: 1 cm. (j) Representative Oil Red O staining of livers from control and Opa1ΔAT mice (n = 7/genotype). Red indicates lipid deposits. Inset is magnified 2X. Bar: 20 µm. (k) Average±SEM of blood glucose levels following an i.p. glucose tolerance test (GTT) performed on control and Opa1ΔAT mice (n = 5-6). **, p < 0.01; *, p < 0.05 in a two-tailed Mann-Whitney U test. (l) Average±SEM of blood glucose levels following an i.p. insulin tolerance test (ITT) performed on control and Opa1ΔAT mice. **, p < 0.01 in a two-tailed Mann-Whitney U test. In panels c, d, g, h dots represent the individual measurements, boxes mean ± SEM, whiskers the 10th-90th percentile.
