Extended Data Fig. 8: Adipocyte Opa1 deletion impairs BAT thermogenic activity.
(a) Top: Gross morphology of representative BAT from Wt and Opa1ΔATmice. Scale bar: 1 cm. Bottom: representative Hematoxylin-Eosin staining of BAT mice of the indicated genotype. Scale bar: 50 µm. (b) Representative fluorescence images of Wt and Opa1ΔAT BAT sections immunostained for UCP1 (red) and with DAPI (blue) (n = 3/genotype). Scale bar: 50 µm. (c) Box-dot plots of UCP1 staining intensity in BAT sections of Wt and Opa1ΔAT mice (n = 27 sections over 3 mice/genotype). Dots represent individual sections, boxes SEM, center line mean, whiskers the 10th-90th percentile. ***, p = 2.6×10−11 in a two-tailed Mann-Whitney U test. (d) Fold change (Opa1ΔAT vs. Wt) of the indicated genes in Wt and Opa1ΔAT BAT (n = 3 mice/genotype). Centre line: mean; whiskers: SEM. *, p = 0.049 for Opa1, Pparγ2, CideA, Tfam, Pgc1α and p = 0.043 for Ucp1 in a Kruskal-Wallis ANOVA test. (e) Representative pseudocolored dorsal view images of IR thermography of conscious male Wt and Opa1ΔATmice. Images were collected at room temperature (RT, 22–23 °C) and 1 h after cold exposure (4 °C). (f) Average±SEM of body skin temperature measured at the indicated times in experiments as in (e) (n = 4 mice/genotype). *, p < 0.05 in a two-tailed Mann-Whitney U test.
