Extended Data Fig. 2: Heat production and ANCOVA analysis of heat production in Opa1tg mice.
(a) Plot of ANCOVA predicted mean energy expenditure for total body weight (BW) values in Opa1tg and Wt mice. Data are from experiments in Fig. 3h (n = 7 mice/genotype). Multiple linear regression analysis of the impact of body mass covariate on energy expenditure was calculated using the MMPC Web Tool at the MMPC Energy Expenditure Analysis Page. Overall p value refers to significance of the ANCOVA-adjusted comparison when BW was taken over both Opa1tg and Wt groups. Group p-value refers to significance of the ANCOVA-adjusted comparison when BW was taken over the two individual Opa1tg and Wt groups. (b, c) Average±SEM of heat release normalized by lean mass (LM, b) or fat mass (FM, c) in light and dark (indicated as gray vertical stripes on the graph) cycles recorded in male mice of the indicated genotypes at 24 °C and at the indicated temperature. n = 7 mice/genotype. ***, p = 4.1×10−4 (basal heat production at 24 °C) and 9.7×10-5 (at 18 °C) in one-way repeated measures ANOVA.
