Extended Data Fig. 3: Validation of Cre-mediated recombination in brain vascular endothelial cells. | Nature Metabolism

Extended Data Fig. 3: Validation of Cre-mediated recombination in brain vascular endothelial cells.

From: Insulin signalling in tanycytes gates hypothalamic insulin uptake and regulation of AgRP neuron activity

Extended Data Fig. 3

a. PCR for wildtype insulin receptor gene (IR) and Exon 4 deleted insulin receptor allele (IR del) in hypothalamus, cortex, muscle, liver, BAT and WAT of IRfl/fl Slco1c1-CreERT2wt/wt (wt) and IRfl/fl Slco1c1-CreERT2tg/wt mice (tg) 8 weeks post tamoxifen treatment (n = 4/4). L – Gene Ruler 1 kb Plus DNA ladder. b. ZsGreen reporter protein expression in hypothalamus, cortex, brain vasculature, skeletal muscle, liver, BAT, WAT and pancreas of Rosa26|STOP | ZsGreenfl/fl Slco1c1-CreERT2tg/wt mice 2 weeks post tamoxifen treatment. Arrows indicate co-localized ZsGreen signal with lectin positive microvessels. Scale bar: 100 µm, scale bars in insets 30 µm.

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