Extended Data Fig. 4: Reduced insulin uptake and insulin receptor signalling in tanycytes in IR^ΔTan-mice.

a, b. Representative images and quantification of spontaneous fluorescence of fluorescently labelled 488-insulin (250 nmol/kg) 15 min post i.p. injection in IR^mKate2 and IR^ΔTan animals, p = 0.0187. c. Representative images of pAKT immunostaining 15 min post injection of 488-insulin (250 nmol/kg) in IR^mKate2 and IR^ΔTan animal. d-f. Immunostaining of pAKT revealed reduced insulin signalling in basal, lateral tanycytes and in ARC, quantified as mean intensity and positive cells per ARC hemisphere, respectively p(basal) = 0.0237, p(lateral) = 0.0145, p(ARC) = 0.0002. a-f. n = 7/IR^mKate2; n = 5/IR^ΔTan, unpaired, two-tailed Student’s t-test. Data are represented as the mean ± SEM. Scale bar: 100 µm.

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