Extended Data Fig. 6: Tanycyte insulin receptor gates insulin dependent activity of AgRP neurons.
a, c. Fos mRNA expression in Agrp (a) and POMC (c) mRNA expressing cells of the ARC (Fos+ positive cells per ARC hemisphere) in 16h fasted animals, which were further fasted for 1 h (Fasted) or refed for 1 h (Refed) of IRGFP-Tan and IRΔTan mice. Scale bar: 100 µm, Scale bar in insets: 30 µm. b, d. Quantification of Fos and Agrp (b) and POMC (d) neurons (Fos+ positive cells per ARC hemisphere) in 16 h fasted animals, which were further fasted for 1 h (Fasted) or refed for 1 h (Refed) animals revealed increased AgRP neuron activation. For Agrp p(Fasted) = 0.0009, p(Refed) = 0.0036, For POMC p(Fasted) = 0.7436, p(Refed) = 0.5483, unpaired, two-sided Student’s t-test. Data are represented as the mean ± SEM. a, c. Dashed line represent the bottom of the Arcuate nucleus (ARC). Arrows indicate co-localized signal with DAPI and the respective neuronal marker (Agrp or POMC). n = 7/Fasted IRGFP-Tan and IRΔTan; n = 6/Refed IRGFP-Tan and IRΔTan.
