Extended Data Fig. 10: Validation of L10a-GFP tagged ribosome expression and Cre-recombinase mediated splicing of Exon 4 of IR in IR∆Tan-mice. | Nature Metabolism

Extended Data Fig. 10: Validation of L10a-GFP tagged ribosome expression and Cre-recombinase mediated splicing of Exon 4 of IR in IR∆Tan-mice.

From: Insulin signalling in tanycytes gates hypothalamic insulin uptake and regulation of AgRP neuron activity

Extended Data Fig. 10

a. Expression of GFP-tagged ribosomes in control L10a-GFPtg/wt and experimental IRfl/fl L10a-GFPtg/wt-mice 3 weeks post i.c.v. injection of AAV-Dio2-Cre virus. Endogenous GFP (EGFP, green) signal was amplified via immunostaining (GFP, red). n = 3/group. Scale: 100 µm. b. ggShashimi visualization of splicing events and read coverage of insulin receptor gene Insr (Chromosome 8: 3,172,061-3,329,617) by displaying exon spanning reads in pulldowns of L10a-GFPtg/wt (control) and IRfl/fl L10a-GFPtg/wt (IRΔTan) mice. For visual clarity, we require a minimum of 10 exon spanning reads to be displayed. The plots show a splicing event in exon 4 of the IRΔTan condition as well as a lower read coverage compared to wild-type.

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