Fig. 6: Tanycyte IR is required for ghrelin access in ARC.

a, Food intake in random-fed IR^mKate2 control animals after NaCl (0.9%) or ghrelin injection (i.p., 1 mg kg⁻¹) over the 4-h measurement time. Two-way ANOVA P = 0.0002, P(1 h) = 0.013, P(2 h) = 0.0009, P(4 h) < 0.0001 (two-way ANOVA, Šídák post hoc test). b, Food intake 4 h post treatment with NaCl (0.9%) or ghrelin (i.p., 1 mg kg⁻¹) in IR^mKate2 control animals, P = 0.0001 (paired, two-tailed Student’s t-test). a,b, n = 12/IR^mKate2. c, Mean food intake in random-fed IR^ΔTan animals after saline or ghrelin injection (i.p., 1 mg kg⁻¹) over the 4-h measurement time. d, Food intake 4 h post treatment with NaCl or ghrelin injection (i.p., 1 mg kg⁻¹) in IR^ΔTan animals. c,d, n = 13/IR^ΔTan. a–d, Data are represented as the mean ± s.e.m. * - P <0.05, ** - P <0.01, *** - P <0.001, **** - P <0.0001.

Source data